ISSN:
1432-0886
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract An unusual form of staining was observed as a result of heat treatment: chromosome contour became easily recognizable. Chromosome contour is optimally delineated by heat treatment of metaphase preparations in 0.06 M phosphate buffer, pH 6.8–7.0 at 95° C for 10 min, and subsequent staining with Heidenhain's hematoxylin. This result is obtained by a process similar to that in which Giemsa has been reported to stain particular foci considered to represent selective staining of constitutive heterochromatin. Mordanting with 3% ferrous ammonium sulfate produced maximum staining. However, hematoxylin (Harris) also stained the periphery of chromosomes. A transitional temperature existed between 70° C and 80° C in which contour became evident. When metaphase preparations were heated at 90–95° C, contour occurred on some chromosomes within 3 min and was demonstrated in 100% of the chromosomes analysed after 10 min of heat treatment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00326183
