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A method for the microinjection and culture of protoplasts at very low densities (1985)

Lawrence, W. A. ; Davies, D. R.

Springer

Plant cell reports 4 (1985), S. 33-35

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ISSN: 1432-203X

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A method has been developed which allows the recovery of calli from a high proportion of individual, injected, mesophyll protoplasts of Nicotiana tabacum c.v. Xanthi. A small drop of low melting point agarose is used both to hold protoplasts during microinjection and for their subsequent culture in feeder dishes. The feeder dishes consist of "beads" of protoplasts at a high density set in agarose to "feed" the infected protoplasts across a liquid medium. The method has been used successfully both with normal protoplasts and protoplasts from which the vacuole has been removed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00285500

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