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Induction and in vitro culture of soybean crown gall tumors (1983)

Pedersen, H. C. ; Christiansen, J. ; Wyndaele, R.

Springer

Plant cell reports 2 (1983), S. 201-204

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ISSN: 1432-203X

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Induction of crown galls on 4–6 week old soybean (Glycine max (L.) Merr.) plants cultured in growth chambers was obtained with Agrobacterium tumefaciens strains C58, T37 and ACH5. The crown galls were isolated and cultured in vitro as sterile callus and liquid suspension cultures. Transformation was tested by opine tests and by molecular hybridization of restricted cell DNA with T-DNA fragments. Protoplasts were isolated from suspension cultures. Transformed protoplasts regenerate cell walls, divide and form calli without an exogenous supply of hormones.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00270104

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