ISSN:
1432-203X
Keywords:
Key words Soybean chlorotic mottle virus
;
Agrobacterium-mediated transformation
;
NCR promoter
;
Transgenic rice
;
Transgenic tobacco
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The NCR promoter (PNCR) from soybean chlorotic mottle virus (SoyCMV) was used to express the selectable marker, neomycin phosphotransferase (nptII) gene, in Agrobacterium-mediated transformation of both monocot (rice) and dicot (tobacco) plants. A multi-cloning site for insertion of a gene of interest into the binary vector pTN is located proximal to the right border region of T-DNA. When chimeric genes under the control of other strong promoters were located in a head-to-head orientation to the PNCR-nptII gene, kanamycin-resistant tobacco shoots were generated more efficiently than when using the original pTN vectors. This suggests that the enhancer-like sequences in the promoters adjacent to PNCR may promote expression of the PNCR-nptII gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990000191