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Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time (1999)

Myers, J. M. ; Simon, P. W.

Springer

Plant cell reports 19 (1999), S. 32-36

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ISSN: 1432-203X

Keywords: Key words Allium sativum ; Benzyladenine ; Picloram ; Thidiazuron ; 2 ; 4-D ; 2iP

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A long-term regeneration system for garlic (Allium sativum L.) clones of diverse origin was developed. Callus was initiated on a modified Gamborg's B-5 medium supplemented with 4.5 μM 2,4-D and maintained on the same basal medium with 4.7 μM picloram+0.49 μM 2iP. Regeneration potential of callus after 5, 12 and 16 months on maintenance medium was measured using several plant growth regulator treatments. The 1.4 μM picloram+13.3 μM BA treatment stimulated the highest rate of shoot production. Regeneration rate decreased as callus age increased, but healthy plantlets from callus cultures up to 16-months-old were produced for all clones. Regeneration of long-term garlic callus cultures could be useful for clonal propagation and transformation.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s002990050706

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