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  • 1
    ISSN: 1432-203X
    Keywords: Key words Aluminium uptake ; Brefeldin A ; Cell culture ; Nicotiana tabacum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between cellular growth and aluminium (Al) uptake was examined by applying brefeldin A, a vesicle transport inhibitor, to cultured tobacco (Nicotiana tabacum L. cv. BY-2) cells. Cultured cells almost completely lost the capacity for Al uptake when pre-incubated for 1–3 h in a minimal medium. Pre-incubation also diminished subsequent growth in a culture medium. However, competency for Al uptake (20 μm, pH 4.5) was sustained in a dose-dependent and reversible manner when cells were treated with brefeldin A (10 μm), an inhibitor of Golgi-mediated secretion, prior to and during the incubation in minimal medium.
    Type of Medium: Electronic Resource
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