ISSN:
1432-203X
Keywords:
Abbreviations: 2,4‐D = 2,4‐dichlorophenoxyacetic acid; BA = N6‐benzyladenine; GM = germination medium; GMVIT = germination medium with vitamins; GUS =β‐glucuronidase; Kin = kinetin (N6‐furfurylaminopurine); NAA =α‐naphtaleneacetic acid; NPT II = neomycin phosphotransferase II; PCR = Polymerase Chain reaction; T‐DNA = transfer‐DNA; X‐glucuronide = 5‐bromo‐4‐chloro‐3‐indolyl β‐D‐glucuronide
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary. A transfornmation system was developed for Artemisia annua L. plants. Leaf explants from in vitro grown plants developed callus and shoots on medium with 0.05 mg/L naphtaleneacetic acid and 0.5 mg/L N6‐benzyladenine after transformation with the C58Cl RifR (pGV2260) (pTJK136) Agrobacterium tumefaciens strain. A concentration of 20 mg/L kanamycin was added in order to select transformed tissue. Kanamycin resistant shoots were rooted on naphtaleneacetic acid 0.1 mg/L. Polymerase chain reactions and DNA sequencing of the amplification products revealed that 75% of the regenerants contained the foreign genes. 94% of the transgenic plants showed a β‐glucoronidase‐positive response.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050151
