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  • 1
    Electronic Resource
    Electronic Resource
    Expression of the cloned endo-1,3-1,4-β-glucanase gene of Bacillus subtilis in Saccharomyces cerevisiae (1984)
    Springer
    Current genetics 8 (1984), S. 471-475 
    Details
    ISSN: 1432-0983
    Keywords: β-Glucanase ; Expression ; Heterologous DNA ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A cloned endo-1,3-1,4-β-glucanase gene from the Gram-positive bacterium B. subtilis has been located by deletion analysis on a 1.4 kb PvuI-ClaI DNA fragment. This gene has been sub-cloned in the yeast LEU2 vector pJDB207 to produce a hybrid plasmid designated pEHB9. pEHB9 has been transformed to S. cerevisiae and shown to direct the synthesis of an endo-1,3-1,4-β-glucanase in yeast. The β-glucanase activity was low and could only be detected in crude cell extracts of yeast harbouring pEHB9.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00433914
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