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A method to sharply delimit a yeast nuclear gene in a cloned DNA fragment (1982)

Faye, Gérard ; Simon, Michel

Springer

Current genetics 6 (1982), S. 159-162

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ISSN: 1432-0983

Keywords: Saccharomyces cerevisiae ; Transformation ; Gene subcloning

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary We have developped a procedure to delimit the boundaries of a cloned gene carried on a DNA fragment as large as 4 to 5 kilobases. The method consists in the following. Two series of limit digest products generated with a tetranucleotide recognition sequence endonuclease and originating from either of the two ends of this DNA segment are tested for their complementing capacity by yeast transformation. The gene is then delimited by the overlap of the two shortest complementing fragments.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00435216

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