ISSN:
1432-0983
Keywords:
Keywords Kluyveromyces lactis
;
Mitochondrial release factor
;
MRF1
;
Peptide chain termination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We report the isolation and characterization of the Kluyveromyces lactis MRF1 gene encoding mitochondrial peptide chain release factor mRF-1. Over-expression of the KlMRF1 gene has a strong antisuppressive effect in a Saccharomyces cerevisiae mitochondrial nonsense suppressor strain. Inactivation of KlMRF1 results in a dual phenotype: most cells die after about 10–13 generations, while a small number of cells exceed this limit. We propose that the lethality is related to a loss of mitochondrial genome integrity. Surviving Klmrf1 cells are able to grow slowly on the non-fermentable substrate glycerol, indicating the existence of a second mitochondrial release factor activity. Our previous comparative analysis of class I release factors is refined by the incorporation of KlmRF-1 and ten recently identified prokaryotic release factor sequences.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050095
