ISSN:
1432-0983
Keywords:
Saccharomyces cerevisiae
;
pso4-1 mutant Sporulation
;
DNA repair
;
Meiotic recombination Induced mutagenesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have evaluated the effect of the Saccharomyces cerevisiae pso4-1 mutation in sporulation and DNA repair during meiosis. We have found that pso4-1 cells were arrested in an early step of meiosis, before premeiotic DNA synthesis, and hence did not produce spores. These results suggest that the PSO4 gene may act at the start point of the cell cycle, as do some SPO and CDC genes. The pso4-1 mutant cells are specifically sensitive to 8-MOP- and 3-CPs-photoinduced lesions, and are found to be severely affected in meiotic recombination as well as impaired in the mutagenic response, as previously described for mitosis. This means that the PSO4 gene is important for the repair 8-MOP-photoinduced lesions, mainly double-strand breaks, and the processing of these lesions into recombinogenic intermediates.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00326150
