ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Three bacterial strains, A, B and C, were isolated from activated sludge as 2-sulfonato-fatty-acid-methyl-ester (α-SFMe)-degrading microorganisms. From the results of morphological, physiological and biochemical studies, and analyses of 16S rRNA gene sequences, isolate A was identified as Agrobacterium tumefaciens while B and C were Pseudomonas putida, respectively. To demonstrate their capability for the ultimate biodegradation of α-SFMe, the degradation kinetics have been investigated using C14-α-SFMe and 2-14C-labeled C16-α-SFMe. The biodegradation was determined by measuring dissolved organic carbon (DOC) and released SO4 2−, in the shake-culture test, and evolved 14CO2 in the modified Organisation for Economic Co-operation and Development (OECD) test. In the shake culture test with C14-α-SFMe, DOC removal was progressive throughout the test. Liberation of inorganic sulfate started after DOC removal and then rapidly increased. During the 14CO2 evolution tests, the mineralization of radiolabeled carbon started quickly and reached about 80% of the initially added radioactivity at the end of the tests. The results obtained indicated that all of the isolates had the capability for ultimately degrading α-SFMe through the oxidation of the alkyl carbons and desulfonation (cleavage of the C-S linkage).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00172843
