ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Pseudomonas cepacia was cultivated on salicylate as sole carbon and energy source in a fed-batch culture. On-line measurement of salicylate concentration was carried out using a filtration system. Cell-free permeate was passed through a flow-through spectrophotometer. Measurements were carried out at 325 nm. A Proportional-Integral controller was used for regulating the feed rate around a basic dosage scheme to maintain a constant salicylate concentration of 0.2 g/l in the broth. The cell mass concentration increased from less than 1 g dry weight (dw)/l to 12 g dw/l in less than 6 h, and the yield coefficient was 0.4 g dw/g salicylate. The intracellular enzymatic activity of salicylate hydroxylase was virtually unchanged during the fed-batch cultivation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00242938
