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Semi-continuous ligninase production using foam-immobilised Phanerochaete chrysosporium (1987)

Kirkpatrick, N. ; Palmer, J. M.

Springer

Applied microbiology and biotechnology 27 (1987), S. 129-133

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ISSN: 1432-0614

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Summary The immobilisation of Phanerochaete chrysosporium in cubes of polyurethane foam enables ligninase to be produced on a semi-continuous basis. At each successive harvest, cultures are purged with oxygen and ligninase activity induced with veratryl alcohol. Using 200 ml of a five-fold dilution of the batch culture medium in 1l flasks, harvests of ligninase with the same apparent protein profile by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) are obtained every 24 to 48 h. It is also possible to store cubes of foam containing pellets of P. chrysosporium so that further yields of ligninase comparable to fresh cultures can be produced within 48 h when desired.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00251934

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