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Protein determination of cells immobilized in cross-linked synthetic gels (1982)

Freeman, A. ; Blank, T. ; Aharonowitz, Y.

Springer

Applied microbiology and biotechnology 14 (1982), S. 13-15

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ISSN: 1432-0614

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Summary An assay for the determination of the protein content of whole cells immobilized in cross-linked synthetic gels was developed. The assay is based on a three step procedure: a) methanol dehydration, b) protein extraction by 1.0 M alkali at 125°C c) colorimetric assay of the extracted protein according to Bradford's procedure (Bradford M. M. (1976), Anal. Biochem. 72:248–254). The procedure worked out was found adequate for the determination of the protein content of microbial cells immobilized in synthetic and native polymer-gel-systems.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00507997

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