ISSN:
1618-2650
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The fluorescence intensity of 5(6)-carboxyfluorescein (CF) was decreased by addition of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The reaction inside a liposome containing CF and HRP on addition of H2O2 was measured fluorometrically after destruction of the liposome with Triton X-100. The reaction efficiency was higher than that without liposome because CF and HRP were concentrated inside the latter. The determination of H2O2 can be performed with a smaller amount of HRP by liposome encapsulation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00323636