ISSN:
1432-072X
Keywords:
Candida boidinii
;
Yeast
;
Peroxisomes
;
β-Oxidation
;
d-Amino acid oxidase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have studied the induction of peroxisomes in the methylotrophic yeast Candida boidinii by d-alanine and oleic acid. The organism was able to utilize each of these compounds as the sole carbon source and grew with growth rates of μ=0.20 h-1 (on d-alanine) or μ=0.43 h-1 (on oleic acid). Growth was associated with the development of many peroxisomes in the cells. On d-alanine a cluster of tightly interwoven organelles was observed which made up 6.3% of the cytoplasmic volume and were characterized by the presence of d-amino acid oxidase and catalase. On oleic acid rounded to elongated peroxisomes were dominant which were scattered throughout the cytoplasm. These organelles contained increased levels of β-oxidation enzymes; their relative volume fraction amounted 12.8% of the cytoplasmic volume.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00248431
