ISSN:
1432-2242
Keywords:
Key words Molecular markers
;
RFLP
;
RAPD
;
Genetic linkage map
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The F2 generations from two maize crosses were used to compare the ability of RAPD and RFLP marker systems to create a genetic linkage map. Both RFLPs and RAPDs were shown to provide Mendelian-type markers. Most of the RFLPs (80%) could be placed with a good level of certainty (LOD〉4) on the genetic linkage map. However, because of their dominant nature, only between 37% and 59% of the RAPDs could be placed with such a LOD score. The use of combined data from RFLPs and RAPDs increases the level of information provided by RAPDs and allows the creation of a combined RFLP/RAPD genetic linkage map. Thus, the RAPD technique was found to be a powerful method to provide improved probes coverage on a previously created RFLP map and to locate markers linked to chromosomal regions of interest.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050322
