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Cultivar identification in T. aestivum using highly polymorphic RFLP probes (1993)

Vaccino, P. ; Accerbi, M. ; Corbellini, M.

Springer

Theoretical and applied genetics 86 (1993), S. 833-836

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ISSN: 1432-2242

Keywords: T. aestivum ; RFLP ; Fingerprint

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Two probes, specific for HMW-glutenins and γ-gliadins have been used to identify 50 common wheat Italian cultivars, most of which are closely related, and four common wheat cultivars originating outside Italy. The probes revealed complex polymorphic patterns; three probe/enzyme combinations had the necessary sensitivity for the identification of all 54 cultivars. As already shown for potato and barley, the use of four-cutter restriction enzymes and polyacrylamide gels proved particularly useful for detecting polymorphism.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00212609

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