ISSN:
1432-2242
Keywords:
Daucus
;
Protoplast fusion
;
Cybrids
;
Mitochondrial DNA recombination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Protoplasts of Daucus capillifolius isolated from a suspension culture (chromosome number above 60) were X-irradiated over lethal dose (60 krad) just prior to fusion. Protoplasts from D. carota cell line (chromosome number 17) were treated with 15 mM iodoacetamide and fused with the X-irradiated protoplasts. Putative cybrid plants were regenerated on Murashige and Skoog medium (MS) lacking 2,4-D. The regenerated plants possessed chromosome numbers of 17 (2n−1) or 34 (4n−2) and an identical leaf morphology to D. carota. Their mitochondrial DNAs (mtDNAs) were analysed with restriction endonucleases. Novel restriction fragments, not present in mtDNA digests from both parents, were observed in mtDNAs of regenerated plants. These results indicate successful formation of cybrids between D. capillifolius and D. carota by protoplast fusion.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00247552
