ISSN:
1573-904X
Keywords:
iontophoresis
;
electroosmosis
;
transdermal delivery
;
mannitol
;
permselectivity
;
skin isoelectric point
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Purpose. To evaluate the transport number of Na+, and the isoelectric point, of two skin membranes frequently used for iontophoreticin vitroresearch. Methods. Na+ transport numbers were determined by the Hittorf method or by the measurement of membrane potential. The skin isoelectric point was deduced from the electroosmosis of mannitol (a polar non-electrolyte) as a function of pH. Results. The Na+ transport number across porcine skin, like that for hairless mouse, indicated a modest cation permselectivity. Consistent with this observation, the isoelectric points of porcine and hairless mouse skin were determined to be in the ranges of 3.5−3.75 and 4.5−4.6, respectively. That is, at physiological pH, both of these model membranes supports a net negative charge. Conclusions. The permselective properties of porcine and hairless mouse skin are similar (but with the porcine membrane having apparently fewer basic or more weakly-acidic groups than that of the mouse) and consistent with the characteristics, which have been deduced elsewhere, of human skin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1011909623019
