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(CAC)5 detects DNA fingerprints and sequences homologous to gene transcripts in rice (1994)

Gupta, V. S. ; Ramakrishna, W. ; Rawat, S. R. ; [et al.]

Springer

Biochemical genetics 32 (1994), S. 1-8

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ISSN: 1573-4927

Keywords: rice ; simple repetitive sequence ; DNA fingerprinting ; (CAC)5 ; poly(A)+ transcripts

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Abstract In the present report the potential of (CAC)5 is demonstrated for DNA fingerprinting in rice. Based on the fingerprint data, (CAC)5 was estimated to differentiate up to 6.3×108 genotypes of rice. In a search for homologous sequences in rice transcripts, four oligonucleotide probes, namely, (CAC)5, (GATA)4, (GACA)4, and (TG)10, were used. Among these probes, (CAC)5 showed hybridization to total rice RNA in a tissue-specific manner; while a band at 1.2 kb was common in both seed and leaf RNA, there were additional prominent bands at 1.9 and 2.3 kb in seed and leaf RNA, respectively. When (CAC)5 was hybridized to poly(A)+ RNA, in addition to a band at 1.2 kb in seed and leaf, hybridization was observed only in seed to heterogeneously sized RNAs.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00557234

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