ISSN:
1573-4927
Keywords:
Drosophila
;
sulfite oxidase
;
molybdenum
;
MoCo
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract The relationship between sulfite oxidase (SO) and sulfite sensitivity inDrosophila melanogaster is addressed. Significant improvements to the SO assay have provided an investigative tool which can be applied to further studies of this molybdoenzyme. Using the second-instar larval stage ofD. melanogaster, we have shown a direct relationship between measured levels of sulfite oxidase activity and the organism's ability to withstand a sulfite challenge. Implementation of a sulfite-testing procedure confirmed the documented instability of sulfite in solution and may explain some of the conflicting results reported in the SO literature. Results of the tungstate-addition experiments confirm thatDrosophila SO is a molybdoenzyme and its activity was shown to be governed by three of the four loci known to affect more than one molybdoenzyme. The ability ofD. melanogaster to withstand the application of exogenous sulfites is shown to be dependent on sulfite oxidase activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00553456
