ISSN:
1573-4927
Keywords:
Mus musculus
;
chromosome 8
;
gene order
;
hybrid enzyme
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract A unique recombination is described between (Es-1, Es-6) and (Es-9, Es-22) within gene cluster 1 of the esterase gene region on chromosome 8 of the house mouse. This recombination established the gene orderEs-1—Es-6—(Es-9, Es-22)—Got-2. A further 73 recombinations, from a total of 911 backcrosses, had taken place between cluster 1 and cluster 2. A distance between the clusters of 8.01±0.90% was calculated; the genes within the clusters appeared more tightly linked than previously assumed. ES-20 behaved anomalously following the recombination within cluster 1: homozygous descendants of the recombinant expressed a new form of ES-20.In vitro incubation of purified ES-6A3 and ES-9A generated ES-20A, revealing this esterase to be a hybrid of different cluster 1 gene products,Es-9 and possiblyEs-6. This result satisfactorily accounted for the genetic finding, as well as a range of biochemical properties of ES-20.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02399407
