ISSN:
1573-9368
Keywords:
transgenic mice
;
reporter genes
;
gene expression
;
histochemical marker
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The human placental alkaline phosphatase (PLAP) gene was analysed for its utility as a histochemically detectable reporter gene in transgenic mice. A reporter gene was made by linking the PLAP structural gene to an enhancerpromoter element from the human β-actin gene. This gene was inserted into the mouse genome by transfection of embryonic stem cells, and by microinjection of fertilized eggs. Histochemical staining showed that the transgene was uniformly expressed in four of four stable ES cell lines, and in all ten tissues examined from adult animals from five lines of transgenic mice. Non-transgenic cells did not stain. These results suggest that the human PLAP gene will be of utility in studies requiring phenotypic marking of cells in tissues of mice.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01980211
