ISSN:
1573-5044
Keywords:
biolistics
;
luciferase
;
tissue culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Reports of wheat transformation efficiencies vary from less than 1% to more than 5% for individual experiments. Rarely are negative experiments reported though we estimate that between one in two and one in three of all experiments fail to produce transformed plants. Consequently if transformation efficiencies were calculated from the total number of scutellum bombarded rather than from only those experiments which produced transformed plants there would be a significant fall in reported efficiencies. The use of scutellum-derived material from plants regenerated from scutellum callus and grown in a controlled environment room significantly reduced the number of experiments failing to produce plants. Though there is a small but significant increase in transformation efficiencies for individual experiments, the recovery of plants, as a direct consequence of the reduction in the number of failed experiments, increases nearly 350%, from 4.8 plants/1000 scutella from seed gown plants bombarded to 17 plants/1000 embryos bombarded from tissue culture regenerated plants. There appears to be no additional gains to be made from plants which are cycled through tissue culture more than one time.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006344615666
