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Agrobacterium tumefaciens-mediated transformation of Mentha spicata and Mentha arvensis (1999)

Diemer, F. ; Caissard, J.C. ; Moja, S. ; [et al.]

Springer

Plant cell, tissue and organ culture 57 (1999), S. 75-78

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ISSN: 1573-5044

Keywords: Agrobacterium tumefaciens ; genetic transformation ; GUS ; Mentha spicata ; Mentha arvensis

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The stable integration of GUS and NPTII genes in Mentha arvensis and M. spicata has been achieved by Agrobacterium tumefaciens-mediated gene transfer. Transformation assays were performed by cocultivating plant leaf disks with either GV2260/GI or EHA105/MOG Agrobacterium strains. Transgenic plants were selected on medium containing 150 mg l−1 kanamycin. Transgene presence and structure was studied by the use of PCR analysis and Southern blot hybridization. Transgene expression was evaluated by RT-PCR and transgene product activity by a histoenzymatic GUS assay.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1006280521212

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