ISSN:
1573-5044
Keywords:
accessory chromosomes
;
Compositae
;
cytogenetics
;
hairy roots
;
polyploidy
;
supernumerary chromosomes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The chromosome behaviour has been compared in three Crepis capillaris callus culture lines and the roots regenerated from these calli. The calli were obtained from explants derived from plants without and with two B chromosomes and the hairy roots were obtained from plants transformed with Agrobacterium rhizogenes. Cytological studies demonstrated that the presence of additional DNA as B chromosomes or as T-DNA had an influence on the numerical and structural variability of the standard chromosome in long-term callus cultures and in regenerated organs. The callus with two B chromosomes displayed higher levels of polyploidyzation than callus without B chromosomes. The roots regenerated from both these calli were only diploid, while roots regenerated from transformed callus were also polyploid.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005727529303
