ISSN:
1573-5044
Keywords:
plant regeneration
;
protoplast
;
somatic embryo
;
suspension
;
Triticum aestivum
;
wheat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We describe the early formation of somatic embryos followed by plant regeneration from protoplasts isolated from an embryogenic wheat cell suspension, which was initiated from small granular (0.2 to 1 mm in size) embryogenic calli. These granular calli formed embryogenic cell suspensions within 20 days in liquid culture, and were selected gradually from young inflorescence-derived nodular embryogenic calli of the winter wheat cv. Kehong 1041. The division frequency of protoplasts was 11 to 16%, and the frequency of differentiation into plants was about 0.001% (number of plants formed divided by the total number of protoplasts plated). About 20% of somatic embryos present in the culture formed directly from protoplast-derived cells within 15 days of cultures.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00039918
