ISSN:
1573-5044
Keywords:
cell culture
;
Daucus carota
;
2-deoxy-D-glucose
;
invertase
;
selection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Variant carrot (Daucus carota L.) cell lines resistant to the growth inhibitory effects of the glucose analogue 2-deoxy-D-glucose (dGlc) were isolated utilizing a feeder plate technique. Growth of sensitive cells was less than 7.5% of controls on medium supplemented with 3.0 mM dGlc, whereas resistant variants achieved growth ranging from 15% to 70% of that in controls. Increased levels of acid invertase activity in variant cell lines in response to dGlc in the culture medium, together with decreased sensitivity of the acid invertase enzyme (EC 3.2.1.26) to dGlc, is proposed as one of several potential mechanisms contributing to the observed dGlc resistance.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00042186
