ISSN:
1573-5079
Keywords:
flavodoxin
;
ferredoxin
;
cyanobacteria
;
thylakoids
;
photosynthesis
;
Photosystem I
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Flavodoxin from the cyanobacterium Anabaena PCC 7119 has been shown to mediate, under illumination, the transfer of electrons from the thylakoidal membranes that were isolated from the same organism, to both the enzyme ferredoxin-NADP+ reductase and cytochrome c. Chemical cross-linking of ferredoxin or flavodoxin to the photosynthetic membranes provides a preparation that is active in cytochrome c photoreduction without the addition of external protein carrier. NADP+ photoreduction, albeit diminished, was observed only after addition of exogenous electron carrier protein. Immunoblotting analysis of the chemical adduct reveals that flavodoxin binds to a 10 kDa polypeptide subunit in the cyanobacterial Photosystem I which appears to act as its physiological partner in the electron transfer process.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00015059
