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A Method for Isolation of Total RNA from Fruit Tissues of Banana (1998)

Liu, Jun-Jun ; Goh, Chong-Jin ; Loh, Chiang-Shiong ; [et al.]

Springer

Plant molecular biology reporter 16 (1998), S. 87-87

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ISSN: 1572-9818

Keywords: banana ; fruit tissue ; Musa sp. ; RNA isolation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract We describe a rapid and efficient method for isolation of total RNA from banana fruit tissues. The RNA was extracted with a high ionic strength buffer at room temperature. The proteins, genomic DNA and secondary metabolites in the extract were then removed by precipitation with pre-cooled potassium acetate and repeated phenol/chloroform/isoamyl alcohol extractions. The RNA was recovered by ethanol precipitation. It was relatively free of ribonucleases and was suitable for RT-PCR and northern blot analysis. The procedure can be completed in less than 4 hours.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1007492421119

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