ISSN:
1573-5028
Keywords:
cysteine proteinase
;
gene expression
;
leaf senescence
;
Zea mays
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Sequence analysis of a 1.4 kb clone from a cDNA library of senescing Zea mays leaves reveals an open reading frame for a 360 amino acid protein. Both the DNA and deduced amino acid sequences are highly homologous to the cysteine proteinases oryzain γ and aleurain. Northern analysis demonstrates that the corresponding RNA level increases during natural leaf senescence, seedling germination and in chilling of tolerant maize lines, but decreases in a sensitive line. The mRNA level also decreases in regreening leaves, in dark-induced senescence and in nutrient or water stress. Southern and RFLP analysis provide evidence that the gene has two copies, on chromosomes 2 and 7.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005896713830