ISSN:
1573-5028
Keywords:
Generative cells
;
gene expression
;
Lilium longiflorum
;
pollen
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Metabolic labelling with [35S]-methionine demonstrated that generative cells ofLilium longiflorum possess their own set of mRNA and are capable of synthesising proteins independently from the vegetative cell. The isolated generative cells synthesised ten proteins, of which six were unique to these specialised cells. Isolation of generative cells from pollen grains after [35S]-methionine labelling resulted in an identical protein profile, therefore the synthesis of these proteins was not due to isolation shock. Addition of cycloheximide, abolished TCA-precipitable counts, whilst actinomycin D had no qualitative effect on the observed protein profile, indicating active translation of pre-existing mRNAs by the generative cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00040727
