ISSN:
1573-5028
Keywords:
glucuronidase reporter
;
heat shock element
;
heat shock promoter
;
seed desiccation
;
transgenic tobacco
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The soybean Gmhsp17.3-B heat shock promoter is developmentally regulated in transgenic tobacco, as indicated by the constitutive expression of a β-glucuronidase reporter in seeds [16]. In this paper, we show that both the heat shock promoter-driven β-glucuronidase activity and the mRNA of the endogenous Nthsp18P gene accumulate coincident with the onset of seed desiccation. Deletions of the soybean Gmhsp17.3-B promoter, encompassing the heat shock element (HSE)-containing regions, revealed a co-localization of sequences responsible for heat induction and developmental expression. Moreover, synthetic HSEs fused to a TATA box sequence had the potential to stimulate the developmental expression of a GUS reporter gene in seeds of transgenic plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00020615
