ISSN:
1573-5028
Keywords:
cab gene
;
expression cloning
;
immunoscreening
;
LHCI
;
photosystem I
;
Pisum sativum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A monoclonal antibody (MAb UB42) is described that binds to thylakoids in pea chloroplasts, as shown by EM-immunogold labelling. The antibody recognised proteins of ca. 23–29 kDa in western blots of a pea leaf homogenate. A cDNA library was prepared from pea epidermal cells in the vector λZAP II, and immunoscreening of the library with UB42 led to the isolation of a clone, pUB42. This was sequenced and had an open reading frame of 269 codons encoding a predicted polypeptide of 28.9 kDa. The sequence showed extensive homology with three closely related polypeptides belonging to a family of chlorophyll a/b-binding proteins from the light harvesting complex of photosytem I (LHCI). Collectively, the results suggest that MAb UB42 recognises an epitope on the type II chlorophyll a/b-binding protein from LHCI and that clone pUB42 encodes this protein.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00020235
