ISSN:
1573-5028
Keywords:
cytoplasmic male sterility
;
coxI
;
mitochondria
;
membrane protein
;
wheat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Mitochondria derived from Triticum timopheevi have a chimeric gene, orf256, immediately upstream from coxI. Antibodies to a peptide corresponding to a part of the encoded amino acid sequence of orf256 detect a 7 kDa protein on western blots of mitochondrial proteins from cytoplasmic male-sterile (cms) wheat (T. aestivum nucleus, T. timopheevi mitochondria) but not in mitochondrial proteins from T. aestivum, T. timopheevi, or cms plants restored to fertility by introduction of nuclear genes for fertility restoration. The 7 kDa protein appears to serve as a marker for cms wheat. Its occurrence as an integral protein of the inner membrane may indicate a cms effect through an influence on mitochondrial membrane function.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00039566
