ISSN:
1573-5028
Keywords:
gene expression
;
desaturation
;
oil synthesis
;
embryogenesis
;
stearoyl-acyl carrier protein desaturase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The nucleotide sequence is reported for a cDNA containing the entire coding region of a stearoyl-ACP desaturase (EC 1.14.99.6) fromBrassica napus L. cv. Jet neuf. The cDNA was obtained from a library constructed from poly(A)+ RNA purified from embryo tissue. The derived amino acid sequence demonstrates substantial similarity with those from other plant Δ9-desaturases. Comparative RNA-dot blot analyses using the Δ9-desaturase cDNA and a rapessed oleosin cDNA as probes showed that although both these transcripts were seed-specific, they exhibited distinct patterns of temporal regulation. The desaturase message was induced by 25 days after anthesis (DAA), peaking at 45 DAA but decreasing considerably thereafter. In contrast, the oleosin transcript did not increase until 45–50 DAA, reaching a peak much later at about 70 DAA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00029157
