ISSN:
1573-5028
Keywords:
Coix
;
coixin
;
sulfur-rich prolamin
;
storage protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A full-length cDNA clone encoding a sulfur-richCoix prolamin was isolated using a cDNA library constructed from polysomal mRNA prepared from immatureCoix endosperm. The deduced amino acid sequence of the cDNA clone predicted a polypeptide of 194 residues, which shared 64% homology with the 17 kDa β-zein. The mature protein contains the familiar composition of the prolamins and an unusually high content of the sulfur-containing amino acids methionine (11.6%) and cysteine (5.2%).In vitro transcription followed byin vitro translation of the coding region of the pBCX17.9S clone gave rise to a polypeptide with an apparent molecular weight corresponding to the C4 α-coixin. Hydropathy analysis showed that C4 α-coixin is slightly more hydrophobic than β-zein.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00018475
