ISSN:
1573-0603
Keywords:
Angiogenesis
;
Endothelial cells
;
Angiogenesis analysis
;
Electron microscopy
;
Cell culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Determination of the structural characteristics of angiogenesis requires a procedure protective of the forming vascular fibers and the endothelial cell monolayer exhibiting cord formation. This report describes in situ fixation of angiogenic cultures in 96-well plates and the subsequent double embedding processing for electron microscopy. Cross sections of the monolayer are obtained without damage of the incipient capillaries.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00123518