ISSN:
1573-0603
Keywords:
pH
;
NH 4 +
;
BCECF
;
human kidney
;
proximal tubule
;
cell culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary This protocol describes the use of the pH-sensitive, intracellularly trapped dye 2′,7′-bis (2-carboxyethyl), 5 (and -6) carboxyfluorescein (BCECF), to characterize the pH regulating mechanisms in cultured human kidney proximal tubule cells. This is a reliable method for intracellular pH measurements and is applicable to single cells, cell suspensions, and confluent cultures.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02388128