ISSN:
1573-5117
Keywords:
RAPD
;
nile perch
;
Tanganyika
;
endemic
;
genetic differentiation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The genetic differentiation of endemic nile perch (Lates stappersi) populations in Lake Tanganyika was studied using RAPD. DNA was extracted from alcohol stored muscle tissue by a salting method, without organic solvents. Three primers amplified 58 variable DNA fragments from 270 individuals from five localities. The genetic distances of local samples as inverse of bandsharing ranged from 0.097 to 0.312. The population sampled in Kigoma, close to the estuary of the Malagarazi river showed high values of genetic distance in pairwise comparisons with other sampled populations. Principal component analysis separated the main population and the 25 samples from Kigoma with high eigenvalues. Five individuals sampled in Kigoma were united with the main population, as confirmed by significant differences in band frequences. The local population in Kigoma had significantly different frequencies in 24 RAPD bands when compared to the pooled samples of Lates stappersi. No clearly diagnostic fragments were found. The genetic distance (1-F) between the Kigoma population and the united main stock was 0.195. Based on Slatkin's index on private alleles, the level of migration between Kigoma and all other sampling sites united, migration is restricted (Nm = 0.43) and allows genetic separation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1003797700309
