ISSN:
1573-5117
Keywords:
Turbellaria
;
planaria
;
cell culture
;
leucine uptake
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have tested various conditions for the culture of cells dispersed from planarians. The cells were procurred by digestion of planarian tissue in pronase and filtering through a nylon mesh. Using incorporation of L-[3H]leucine into protein as a gauge of cell growth, we found that the optimum salt concentration was about 50% of that for mammalian cells (about 160 mOsm) and that optimum pH was about 8. Sera from several species and a tetrapeptide (Arg-Gly-Asp-Ser, the cell-attachment sequence in fibronectin) greatly increased leucine uptake and extended cell survival up to a period of about two weeks. Various growth factors and some other substances tested had no effect on uptake of leucine, cell morphology, or survival. A few other compounds tested were cytotoxic. None of the experimental media promoted cell proliferation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00027601
