ISSN:
1573-5117
Keywords:
phosphorus
;
reactive phosphorus
;
filtration
;
centrifugation
;
gel chromatography
;
extraction
;
enzymes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In recent research, particulate and dissolved phosphorus components have been separated and characterized on the basis of their physical and chemical properties and partly by their origins. Classical operationally defined monitoring variables (dissolved reactive phosphorus, dissolved unreactive phosphorus and particulate phosphorus) are not congruent with known specific physical or chemical components of phosphorus in natural waters or with their bioavailability. Physical isolation of true particles, colloids and molecules of various sizes is possible at present although it is not recommended for routine use. Chemical characterization of particulate phosphorus is performed mainly by sediment extraction procedures (specialized for inorganic species) and — to a lesser degree — by cell extraction procedures (specialized for organic compounds). The extraction procedures are similar and physical preseparation or alternative procedures (e.g. enzymatic assays) are essential. Smaller colloids and dissolved compounds are physically separated by column chromatography and are often chemically characterized by degradation on the addition of specific enzymes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00024899
