ISSN:
1573-5060
Keywords:
Triticum aestivum
;
wheat
;
electrophoresis system
;
acid polyacrylamide gel
;
gliadins
;
variety identification
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary In this paper we describe the method of wheat gliadin electrophoresis in use at RIVRO, Wageningen. It differs from other techniques mainly by the application of an alternative buffer system, making it possible to polymerise the gels in a buffered alkaline environment and to perform the run at pH 3.1 without extensive buffer changing steps. Advantages are a greater gel reproducibility and the ease of gel handling. Furthermore, a rationalised protein extraction procedure, a cheap shaking system for staining baths and a better (slower moving) tracking dye are described.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00730647
