ISSN:
1573-0778
Keywords:
blastocyst
;
egg cylinder
;
embryogenesis
;
implantation
;
laminin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. α1, β1 and γ1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. α4 and α5 mRNAs were additionally expressed after attachment of blastocysts to culture dishes. α2 and β2 mRNAs started to be expressed at the stage of allantois formation. Among ten laminin chains, only the mRNA encoding components of laminin-5 (α3, β3 and γ2) was missing in embryos up to the stage of allantois formation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008084508489
