ISSN:
1572-9737
Keywords:
cross-amplification
;
microsatellite
;
passerine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Developing species specific microsatellite primers can be avoidedby using existing markers which amplify across species. However,for passerines, such ‘cross-species’ markers are mostly lackingand few guidelines exist for selecting them from the wide rangeof existing markers. Here cross-species amplification tests of 40microsatellite primers in 13 passerine species show an increasein probability of amplification and polymorphism with decreasingphylogenetic distance. Primers which successfully amplified inmany species had a higher chance to be polymorphic. However,since the amplification success, across a broad range of species,of particular primersets remains difficult to predict it iscrucial to identify such markers empirically. Here we describesuch widely applicable bird (passerines) microsatellite markers.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1026587024065
