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A combination of sister chromatid differential staining and giemsa banding (1975)

Pathak, S. ; Stock, A. D. ; Lusby, A.

Springer

Cellular and molecular life sciences 31 (1975), S. 916-918

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ISSN: 1420-9071

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary We report a procedure for combining sister chromatid differential staining and G banding in the same metaphase plate. Mammalian cells in culture are grown in medium containing 5-bromodeoxyuridine for two cell cycles, and conventional air-dried preparations are made. The slides are treated with a trypsin or a urea solution the same way as for regular G banding. This method is simple and fast and provides additional information for cytogeneticists.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02358850

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