ISSN:
0192-253X
Keywords:
mRNA structure
;
initiation of protein synthesis
;
ribosome gel electrophoresis
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
A method is described for the experimental determination of the secondary structure of RNA using enzymatic cleavage data coupled with computer analysis. The structure-specific enzymes S1 nuclease and cobra venom ribonuclease are used to locate nonpaired and basepaired nucleotides, respectively. Computer techniques that utilize the enzymatic susceptibility information to generate a minimum free-energy structure are used to obtain secondary structure models. A second method, using acrylamide-agarose gel electrophoresis, is described for the determination of the relative protein synthesis initiation rates of endlabeled eukaryotic mRNAs. These methods are applied to the rabbit globin mRNAs as an example of a general approach for relating mRNA structure and function. A discussion of the role of messenger RNA structure in the regulation of translation is included with an emphasis on studies of development.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/dvg.1020040408
