ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
A number of reactions which lead to the removal of 3′-phosphate and 5′-phosphate ends of DNA have been detected in extracts of E. coli. Two new enzymes which attack the ends of DNA chains have been purified from phage-infected cells. One enzyme removes only 3′-phosphate termini specifically from DNA, whereas the other removes only 5′-phosphate ends from both DNA and RNA. Synthetic reactions occurring at ends of DNA have also been detected. In phage-infected extracts, 5′-hydroxyl polynucleotide kinase catalyzes the phosphorylation of 5′-hydroxyl-terminated polynucleotides. Another phage-induced enzyme, in the presence of ATP and deoxynucleoside triphosphates, leads to the conversion of 5′-32P-labeled DNA to a form resistant to alkaline phosphatase. This enzyme preparation also catalyzes the conversion of the Hershey circle form of lambda DNA to a covalent form. Enzyme fractions purified from E. coli K12 also catalyze the latter reaction.
Additional Material:
11 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1040700413